Jump to the main content block

Development of therapeutic antibodies against dengue disease

Development of therapeutic antibodies against dengue disease

Dengue fever is a mosquito-borne viral disease; however, the ideal antiviral drugs have not fully completed. Therapeutic antibodies have the characteristics of high specificity, neutralization and low immune rejection, and have been used in cancer, immune diseases or infectious diseases. Antibodies (Abs) against the structural proteins of dengue virus have the risk of antibody-dependent enhancement which may cause more serious diseases and thus restrict the development of drugs. Relatively, Abs against nonstructural protein 1 (NS1) do not have this risk; therefore, there is more attention on the development of therapeutics anti-NS1 Abs. We prepared a humanized anti-NS1 monoclonal Abs and demonstrated their protective efficacy in cell-based and animal models. We hoped that this monoclonal antibody can be commercialized in the future and provide a novel strategy for the treatment of dengue disease.


  Dengue virus (DENV) which infects up to 400 million people annually in tropical and subtropical areas manifests various disease symptoms, ranging from self-limiting fever to life-threatening severe dengue which often requires care at a hospital. To date, we still relay on supportive care for dengue patients without effective medicine. Recently, DENV nonstructural protein 1 (NS1) has been shown the key roles in disease pathogenesis, including hemorrhage and vascular leakage. Hence, therapeutic anti-DENV NS1 monoclonal antibodies (mAbs) are a potential strategy to provide protection by blocking the DENV-induced disruption of endothelial integrity. In this study, we generated humanized anti-NS1 mAbs and evaluated the protective efficacy in vitro and in vivo. In the DENV infection mouse model, we demonstrate that humanized anti-NS1 mAbs have therapeutic benefits not only in decreasing viral load and NS1 concentration but also reducing DENV-induced prolonged bleeding time and skin hemorrhage. In vitro mechanistic studies showed a reduction of NS1-induced vascular permeability to maintain endothelial integrity and an increase in cytolysis of DENV-infected cells to eliminate viruses. Taken together, humanized anti-NS1 mAb present strong therapeutic potential for development toward clinical use not only in febrile phase but also the critical phase (Figure 1)

Figure 1. Humanized anti-NS1 mAbs is a potential therapeutic agent against dengue disease. This figure is created with Biorender.com.



Shu-Wen Wan


Department of Microbiology and Immunology, College of Medicine



 Trai-Ming Yeh


Department of Medical Laboratory Science and Biotechnology,

College of Medicine


Click Num:

subscribe E-news

Vol.34 NO.3(2022.6)-4 Author information


Shu-Wen Wan NCKU Assistant Professor

Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University

Trai-Ming Yeh NCKU Professor

Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University


Shu-Wen Wan  Assistant Professor


Trai-Ming Yeh NCKU Professor


Research members

Sen-Mao Tien1, Po-Chun Chang1,2, Yen-Chung Lai2,3,4, Yung-Chun Chuang2,4, Chin-Kai Tseng5, Yu-San Kao1, Hong-Jyun Huang1, Yu-Peng Hsiao1, Yi-Ling Liu1, Hsing-Han Lin1,5, Chien-Chou Chu1, Miao-Huei Cheng1, Tzong-Shiann Ho6,7, Chih-Peng Chang1,7, Shu-Fen Ko8, Che-Piao Shen8, Robert Anderson9, Yee-Shin Lin1,7, Shu-Wen Wan1,7, Trai-Ming Yeh4,7

1Department of Microbiology and Immunology, National Cheng Kung University.

2Leadgene Biomedical, Inc.

3Institute of Basic Medical Sciences, National Cheng Kung University.

4Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University.

5SIDSCO Biomedical Co., Ltd.

6Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University.

7Center of Infectious Disease and Signaling Research, National Cheng Kung University.

8Development Center for Biotechnology.

9Department of Microbiology and Immunology, Dalhousie University, Canada.

Key words

Dengue virus, nonstructural protein 1, therapeutic monoclonal antibodies

Research areas

Translational research in biomedicine


Therapeutic efficacy of humanized monoclonal antibodies targeting dengue virus nonstructural protein 1 in the mouse model.

PLoS Pathogens 2022; 18(4): e1010469. https://doi.org/10.1371/journal.ppat.1010469


The Development of Lead-Free Piezoelectric MEMS Triaxial Accelerometer Systemfor Safety Monitoring of Unmanned VehiclesDevelopment of therapeutic antibodies against dengue disease